Development of a rapid, simple and efficient one-pot cloning method for a reverse genetics system of broad subtypes of influenza A virus

The reverse genetics (RG) system of influenza A viruses is well established. However, the conventional sequence-dependent method for cloning influenza genome segments is time-consuming and requires multiple processes (eg. enzyme digestion and ligation) and exhibits low cloning efficiency compared to...

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Pubblicato in:Sci Rep
Autori principali: Choi, Won-Suk, Jeong, Ju Hwan, Lloren, Khristine Kaith S., Ahn, Su Jeong, Antigua, Khristine Joy C., Kim, Young-il, Si, Young-Jae, Baek, Yun Hee, Choi, Young Ki, Song, Min-Suk
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2019
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6549168/
https://ncbi.nlm.nih.gov/pubmed/31165766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-44813-z
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