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Advanced FRET normalization allows quantitative analysis of protein interactions including stoichiometries and relative affinities in living cells
FRET (Fluorescence Resonance Energy Transfer) measurements are commonly applied to proof protein-protein interactions. However, standard methods of live cell FRET microscopy and signal normalization only allow a principle assessment of mutual binding and are unable to deduce quantitative information...
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| 出版年: | Sci Rep |
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| 主要な著者: | , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Nature Publishing Group UK
2019
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6547726/ https://ncbi.nlm.nih.gov/pubmed/31160659 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-44650-0 |
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