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A Novel FRET Approach Quantifies the Interaction Strength of Peroxisomal Targeting Signals and Their Receptor in Living Cells

Measuring Förster–resonance–energy–transfer (FRET) efficiency allows the investigation of protein–protein interactions (PPI), but extracting quantitative measures of affinity necessitates highly advanced technical equipment or isolated proteins. We demonstrate the validity of a recently suggested no...

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Detalhes bibliográficos
Publicado no:Cells
Main Authors: Hochreiter, Bernhard, Chong, Cheng-Shoong, Hartig, Andreas, Maurer-Stroh, Sebastian, Berger, Johannes, Schmid, Johannes A., Kunze, Markus
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7693011/
https://ncbi.nlm.nih.gov/pubmed/33143123
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/cells9112381
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