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Optimization of Protein Entrapment in Affinity Microcolumns using Hydrazide-Activated Silica and Glycogen as a Capping Agent
Several approaches were compared for the entrapment of proteins within hydrazide-activated silica for use in affinity microcolumns and high performance affinity chromatography. Human serum albumin (HSA) and concanavalin A (Con A) were used as model proteins for this work. Items considered in this st...
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| Publicado no: | J Chromatogr B Analyt Technol Biomed Life Sci |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6545136/ https://ncbi.nlm.nih.gov/pubmed/31079009 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2019.05.004 |
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