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Optimization of Protein Entrapment in Affinity Microcolumns using Hydrazide-Activated Silica and Glycogen as a Capping Agent

Several approaches were compared for the entrapment of proteins within hydrazide-activated silica for use in affinity microcolumns and high performance affinity chromatography. Human serum albumin (HSA) and concanavalin A (Con A) were used as model proteins for this work. Items considered in this st...

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Bibliografiska uppgifter
I publikationen:J Chromatogr B Analyt Technol Biomed Life Sci
Huvudupphovsmän: Vargas-Badilla, John, Poddar, Saumen, Azaria, Shiden, Zhang, Chenhua, Hage, David S.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2019
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC6545136/
https://ncbi.nlm.nih.gov/pubmed/31079009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2019.05.004
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