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Nucleus assembly and import in Xenopus laevis egg extract

Xenopus egg extract represents a powerful cell-free biochemical tool for studying organelle assembly and function. Large quantities of cytoplasm can be isolated, and biochemical manipulation of extract composition and cell cycle state is relatively straightforward. In this protocol, we describe the...

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Dettagli Bibliografici
Pubblicato in:Cold Spring Harb Protoc
Autori principali: Chen, Pan, Levy, Daniel L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2018
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6540748/
https://ncbi.nlm.nih.gov/pubmed/29321282
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot097196
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