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Microneedle-based analysis of the micromechanics of the metaphase spindle assembled in Xenopus laevis egg extracts

To explain how micron-sized cellular structures generate and respond to forces we need to characterize their micromechanical properties. Here we provide a protocol to build and use a dual force-calibrated microneedle-based set-up to quantitatively analyze the micromechanics of a metaphase spindle as...

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Detalhes bibliográficos
Main Authors: Shimamoto, Yuta, Kapoor, Tarun M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4117203/
https://ncbi.nlm.nih.gov/pubmed/22538847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.033
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