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Nucleus assembly and import in Xenopus laevis egg extract

Xenopus egg extract represents a powerful cell-free biochemical tool for studying organelle assembly and function. Large quantities of cytoplasm can be isolated, and biochemical manipulation of extract composition and cell cycle state is relatively straightforward. In this protocol, we describe the...

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Publicat a:Cold Spring Harb Protoc
Autors principals: Chen, Pan, Levy, Daniel L.
Format: Artigo
Idioma:Inglês
Publicat: 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6540748/
https://ncbi.nlm.nih.gov/pubmed/29321282
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot097196
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