Detection of circulating extracellular mRNAs by modified small-RNA-sequencing analysis

Extracellular mRNAs (ex-mRNAs) potentially supersede extracellular miRNAs (ex-miRNAs) and other RNA classes as biomarkers. We performed conventional small-RNA-sequencing (sRNA-seq) and sRNA-seq with T4 polynucleotide kinase (PNK) end treatment of total extracellular RNAs (exRNAs) isolated from serum...

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Vydáno v:JCI Insight
Hlavní autoři: Akat, Kemal M., Lee, Youngmin A., Hurley, Arlene, Morozov, Pavel, Max, Klaas E.A., Brown, Miguel, Bogardus, Kimberly, Sopeyin, Anuoluwapo, Hildner, Kai, Diacovo, Thomas G., Neurath, Markus F., Borggrefe, Martin, Tuschl, Thomas
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Clinical Investigation 2019
Témata:
Technical Advance
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6538341/
https://ncbi.nlm.nih.gov/pubmed/30973829
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1172/jci.insight.127317
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