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Programmed gRNA Removal System for CRISPR-Cas9-Mediated Multi-Round Genome Editing in Bacillus subtilis
CRISPR/Cas9 has become a simple and powerful genome editing tool for many organisms. However, multi-round genome editing should replace single-guide RNA (sgRNA) every round, which is laborious and time-consuming. Here, we have developed a multi-round genome editing system in which genome editing and...
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| Published in: | Front Microbiol |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Frontiers Media S.A.
2019
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6536666/ https://ncbi.nlm.nih.gov/pubmed/31164882 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2019.01140 |
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