A Highly Efficient CRISPR-Cas9-Mediated Large Genomic Deletion in Bacillus subtilis

In Bacillus subtilis, large genomic deletions have been carried out for genome reduction, antibiotic overproduction, and heterologous protein overexpression. In view of the eco-friendliness of B. subtilis, it is critical that engineering preserves its food-grade status and avoids leaving foreign DNA...

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Bibliografiske detaljer
Udgivet i:Front Microbiol
Main Authors: So, Younju, Park, Soo-Young, Park, Eun-Hye, Park, Seung-Hwan, Kim, Eui-Joong, Pan, Jae-Gu, Choi, Soo-Keun
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2017
Fag:
Microbiology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5481315/
https://ncbi.nlm.nih.gov/pubmed/28690606
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.01167
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