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A Highly Efficient CRISPR-Cas9-Mediated Large Genomic Deletion in Bacillus subtilis
In Bacillus subtilis, large genomic deletions have been carried out for genome reduction, antibiotic overproduction, and heterologous protein overexpression. In view of the eco-friendliness of B. subtilis, it is critical that engineering preserves its food-grade status and avoids leaving foreign DNA...
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| Publicado no: | Front Microbiol |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Frontiers Media S.A.
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5481315/ https://ncbi.nlm.nih.gov/pubmed/28690606 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.01167 |
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