A Highly Efficient CRISPR-Cas9-Mediated Large Genomic Deletion in Bacillus subtilis

In Bacillus subtilis, large genomic deletions have been carried out for genome reduction, antibiotic overproduction, and heterologous protein overexpression. In view of the eco-friendliness of B. subtilis, it is critical that engineering preserves its food-grade status and avoids leaving foreign DNA...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Front Microbiol
Hauptverfasser: So, Younju, Park, Soo-Young, Park, Eun-Hye, Park, Seung-Hwan, Kim, Eui-Joong, Pan, Jae-Gu, Choi, Soo-Keun
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2017
Schlagworte:
Microbiology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5481315/
https://ncbi.nlm.nih.gov/pubmed/28690606
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.01167
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