A Highly Efficient CRISPR-Cas9-Mediated Large Genomic Deletion in Bacillus subtilis

In Bacillus subtilis, large genomic deletions have been carried out for genome reduction, antibiotic overproduction, and heterologous protein overexpression. In view of the eco-friendliness of B. subtilis, it is critical that engineering preserves its food-grade status and avoids leaving foreign DNA...

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Vydáno v:Front Microbiol
Hlavní autoři: So, Younju, Park, Soo-Young, Park, Eun-Hye, Park, Seung-Hwan, Kim, Eui-Joong, Pan, Jae-Gu, Choi, Soo-Keun
Médium: Artigo
Jazyk:Inglês
Vydáno: Frontiers Media S.A. 2017
Témata:
Microbiology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5481315/
https://ncbi.nlm.nih.gov/pubmed/28690606
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.01167
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