A photo-cleavable surfactant for top-down proteomics

We report the identification of a photo-cleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo) that can be rapidly degraded upon UV irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to SDS and is mass spectrometry (MS)-compatible. Import...

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Udgivet i:Nat Methods
Main Authors: Brown, Kyle A., Chen, Bifan, Guardado-Alvarez, Tania M., Lin, Ziqing, Hwang, Leekyoung, Ayaz-Guner, Serife, Jin, Song, Ge, Ying
Format: Artigo
Sprog:Inglês
Udgivet: 2019
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6532422/
https://ncbi.nlm.nih.gov/pubmed/30988469
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-019-0391-1
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