Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs

We describe a multistep method for endogenous tagging of transcriptionally silent genes in human induced pluripotent stem cells (hiPSCs). A monomeric EGFP (mEGFP) fusion tag and a constitutively expressed mCherry fluorescence selection cassette were delivered in tandem via homology-directed repair t...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Stem Cell Reports
Päätekijät: Roberts, Brock, Hendershott, Melissa C., Arakaki, Joy, Gerbin, Kaytlyn A., Malik, Haseeb, Nelson, Angelique, Gehring, Jamie, Hookway, Caroline, Ludmann, Susan A., Yang, Ruian, Haupt, Amanda, Grancharova, Tanya, Valencia, Veronica, Fuqua, Margaret A., Tucker, Andrew, Rafelski, Susanne M., Gunawardane, Ruwanthi N.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Elsevier 2019
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Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC6522946/
https://ncbi.nlm.nih.gov/pubmed/30956114
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2019.03.001
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