Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs

We describe a multistep method for endogenous tagging of transcriptionally silent genes in human induced pluripotent stem cells (hiPSCs). A monomeric EGFP (mEGFP) fusion tag and a constitutively expressed mCherry fluorescence selection cassette were delivered in tandem via homology-directed repair t...

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Detalhes bibliográficos
Publicado no:Stem Cell Reports
Main Authors: Roberts, Brock, Hendershott, Melissa C., Arakaki, Joy, Gerbin, Kaytlyn A., Malik, Haseeb, Nelson, Angelique, Gehring, Jamie, Hookway, Caroline, Ludmann, Susan A., Yang, Ruian, Haupt, Amanda, Grancharova, Tanya, Valencia, Veronica, Fuqua, Margaret A., Tucker, Andrew, Rafelski, Susanne M., Gunawardane, Ruwanthi N.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6522946/
https://ncbi.nlm.nih.gov/pubmed/30956114
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2019.03.001
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