Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs

We describe a multistep method for endogenous tagging of transcriptionally silent genes in human induced pluripotent stem cells (hiPSCs). A monomeric EGFP (mEGFP) fusion tag and a constitutively expressed mCherry fluorescence selection cassette were delivered in tandem via homology-directed repair t...

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Detaylı Bibliyografya
Yayımlandı:Stem Cell Reports
Asıl Yazarlar: Roberts, Brock, Hendershott, Melissa C., Arakaki, Joy, Gerbin, Kaytlyn A., Malik, Haseeb, Nelson, Angelique, Gehring, Jamie, Hookway, Caroline, Ludmann, Susan A., Yang, Ruian, Haupt, Amanda, Grancharova, Tanya, Valencia, Veronica, Fuqua, Margaret A., Tucker, Andrew, Rafelski, Susanne M., Gunawardane, Ruwanthi N.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Elsevier 2019
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Resource
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC6522946/
https://ncbi.nlm.nih.gov/pubmed/30956114
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2019.03.001
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