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Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs

Summary: We describe a multistep method for endogenous tagging of transcriptionally silent genes in human induced pluripotent stem cells (hiPSCs). A monomeric EGFP (mEGFP) fusion tag and a constitutively expressed mCherry fluorescence selection cassette were delivered in tandem via homology-directed...

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Hoofdauteurs: Brock Roberts, Melissa C. Hendershott, Joy Arakaki, Kaytlyn A. Gerbin, Haseeb Malik, Angelique Nelson, Jamie Gehring, Caroline Hookway, Susan A. Ludmann, Ruian Yang, Amanda Haupt, Tanya Grancharova, Veronica Valencia, Margaret A. Fuqua, Andrew Tucker, Susanne M. Rafelski, Ruwanthi N. Gunawardane
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2019-05-01
Reeks:Stem Cell Reports
Online toegang:http://www.sciencedirect.com/science/article/pii/S2213671119300864
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