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Using the SpyTag SpyCatcher system to label smooth muscle myosin II filaments with a quantum dot on the regulatory light chain

The regulatory light chain (RLC) of myosin is commonly tagged to monitor myosin behavior in vitro, in muscle fibers, and in cells. The goal of this study was to prepare smooth muscle myosin (SMM) filaments containing a single head labeled with a quantum dot (QD) on the RLC. We show that when the RLC...

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Detalhes bibliográficos
Publicado no:Cytoskeleton (Hoboken)
Main Authors: Brizendine, Richard K., Anuganti, Murali, Cremo, Christine R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6520152/
https://ncbi.nlm.nih.gov/pubmed/30861328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cm.21516
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