ClampFISH detects individual nucleic-acid molecules using click chemistry based amplification

Methods for detecting single nucleic acids in cell and tissues, such as fluorescence in situ hybridization (FISH), are limited by relatively low signal intensity and non-specific probe binding. Here we present click-amplifying FISH (clampFISH), a method for fluorescence detection of nucleic acids th...

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Detalhes bibliográficos
Publicado no:Nat Biotechnol
Main Authors: Rouhanifard, Sara H., Mellis, Ian A., Dunagin, Margaret, Bayatpour, Sareh, Jiang, Connie L., Dardani, Ian, Symmons, Orsolya, Emert, Benjamin, Torre, Eduardo, Cote, Allison, Sullivan, Alessandra, Stamatoyannopoulos, John A., Raj, Arjun
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6511493/
https://ncbi.nlm.nih.gov/pubmed/30418432
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.4286
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