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Small RNA-seq: The RNA 5’-end adapter ligation problem and how to circumvent it
The preparation of small RNA cDNA sequencing libraries depends on the unbiased ligation of adapters to the RNA ends. Small RNA with 5’ recessed ends are poor substrates for enzymatic adapter ligation, but this 5’ adapter ligation problem can go undetected if the library preparation steps are not mon...
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| 出版年: | J Biol Methods |
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| 主要な著者: | , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Journal of Biological Methods
2019
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6507418/ https://ncbi.nlm.nih.gov/pubmed/31080843 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14440/jbm.2019.269 |
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