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Adenylylation of small RNA sequencing adapters using the TS2126 RNA ligase I
Many high-throughput small RNA next-generation sequencing protocols use 5′ preadenylylated DNA oligonucleotide adapters during cDNA library preparation. Preadenylylation of the DNA adapter's 5′ end frees from ATP-dependence the ligation of the adapter to RNA collections, thereby avoiding ATP-de...
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| Vydáno v: | RNA |
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| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Cold Spring Harbor Laboratory Press
2016
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4691829/ https://ncbi.nlm.nih.gov/pubmed/26567315 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.054999.115 |
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