Surface patches on recombinant erythropoietin predict protein solubility: engineering proteins to minimise aggregation

BACKGROUND: Protein solubility characteristics are important determinants of success for recombinant proteins in relation to expression, purification, storage and administration. Escherichia coli offers a cost-efficient expression system. An important limitation, whether for biophysical studies or i...

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Udgivet i:BMC Biotechnol
Main Authors: Carballo-Amador, M. Alejandro, McKenzie, Edward A., Dickson, Alan J., Warwicker, Jim
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2019
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6507049/
https://ncbi.nlm.nih.gov/pubmed/31072369
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-019-0520-z
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