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Quantification of Active Apohemoglobin Heme Binding Sites via Dicyanohemin Incorporation

Apohemoglobin (apoHb) is produced by removing heme from hemoglobin (Hb). Unfortunately, preparations of apoHb may contain damaged globins, which render total protein assays inaccurate for active apoHb quantification. Fortunately, apoHb heme-binding sites react with heme via the proximal histidine-F8...

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Bibliografski detalji
Izdano u:Biochemistry
Glavni autori: Pires, Ivan S., Belcher, Donald A., Palmer, Andre F.
Format: Artigo
Jezik:Inglês
Izdano: 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6505700/
https://ncbi.nlm.nih.gov/pubmed/28846391
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.7b00683
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