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Quantification of Active Apohemoglobin Heme Binding Sites via Dicyanohemin Incorporation

Apohemoglobin (apoHb) is produced by removing heme from hemoglobin (Hb). Unfortunately, preparations of apoHb may contain damaged globins, which render total protein assays inaccurate for active apoHb quantification. Fortunately, apoHb heme-binding sites react with heme via the proximal histidine-F8...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Biochemistry
Main Authors: Pires, Ivan S., Belcher, Donald A., Palmer, Andre F.
Format: Artigo
Jezik:Inglês
Izdano: 2017
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6505700/
https://ncbi.nlm.nih.gov/pubmed/28846391
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.7b00683
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