Efficient and unique cobarcoding of second-generation sequencing reads from long DNA molecules enabling cost-effective and accurate sequencing, haplotyping, and de novo assembly

Here, we describe single-tube long fragment read (stLFR), a technology that enables sequencing of data from long DNA molecules using economical second-generation sequencing technology. It is based on adding the same barcode sequence to subfragments of the original long DNA molecule (DNA cobarcoding)...

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Detalhes bibliográficos
Publicado no:Genome Res
Main Authors: Wang, Ou, Chin, Robert, Cheng, Xiaofang, Wu, Michelle Ka Yan, Mao, Qing, Tang, Jingbo, Sun, Yuhui, Anderson, Ellis, Lam, Han K., Chen, Dan, Zhou, Yujun, Wang, Linying, Fan, Fei, Zou, Yan, Xie, Yinlong, Zhang, Rebecca Yu, Drmanac, Snezana, Nguyen, Darlene, Xu, Chongjun, Villarosa, Christian, Gablenz, Scott, Barua, Nina, Nguyen, Staci, Tian, Wenlan, Liu, Jia Sophie, Wang, Jingwan, Liu, Xiao, Qi, Xiaojuan, Chen, Ao, Wang, He, Dong, Yuliang, Zhang, Wenwei, Alexeev, Andrei, Yang, Huanming, Wang, Jian, Kristiansen, Karsten, Xu, Xun, Drmanac, Radoje, Peters, Brock A.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2019
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6499310/
https://ncbi.nlm.nih.gov/pubmed/30940689
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.245126.118
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