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Gain- and Loss-of-Function Screens Coupled to Next-Generation Sequencing for Antibiotic Mode of Action and Resistance Studies in Streptococcus pneumoniae

Two whole-genome screening approaches are described for studying the mode of action and the mechanisms of resistance to trimethoprim (TMP) in the Gram-positive Streptococcus pneumoniae. The gain-of-function approach (Int-Seq) relies on a genomic library of DNA fragments integrated into a fucose-indu...

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Dades bibliogràfiques
Publicat a:Antimicrob Agents Chemother
Autors principals: Gingras, Hélène, Patron, Kévin, Bhattacharya, Arijit, Leprohon, Philippe, Ouellette, Marc
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6496052/
https://ncbi.nlm.nih.gov/pubmed/30783004
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.02381-18
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