Plasma lipoprotein-X quantification on filipin-stained gels: monitoring recombinant LCAT treatment ex vivo

Familial LCAT deficiency (FLD) patients accumulate lipoprotein-X (LP-X), an abnormal nephrotoxic lipoprotein enriched in free cholesterol (FC). The low neutral lipid content of LP-X limits the ability to detect it after separation by lipoprotein electrophoresis and staining with Sudan Black or other...

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Dettagli Bibliografici
Pubblicato in:J Lipid Res
Autori principali: Freeman, Lita A., Shamburek, Robert D., Sampson, Maureen L., Neufeld, Edward B., Sato, Masaki, Karathanasis, Sotirios K., Remaley, Alan T.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The American Society for Biochemistry and Molecular Biology 2019
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6495165/
https://ncbi.nlm.nih.gov/pubmed/30808683
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D090233
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