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Propidium iodide staining underestimates viability of adherent bacterial cells

Combining membrane impermeable DNA-binding stain propidium iodide (PI) with membrane-permeable DNA-binding counterstains is a widely used approach for bacterial viability staining. In this paper we show that PI staining of adherent cells in biofilms may significantly underestimate bacterial viabilit...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Rosenberg, Merilin, Azevedo, Nuno F., Ivask, Angela
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6482146/
https://ncbi.nlm.nih.gov/pubmed/31019274
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-42906-3
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