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Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide

BACKGROUND: Viability staining with SYTO9 and propidium iodide (PI) is a frequently used tool in microbiological studies. However, data generated by such routinely used method are often not critically evaluated for their accuracy. In this study we aim to investigate the critical aspects of this stai...

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Detalhes bibliográficos
Publicado no:BMC Microbiol
Main Authors: Stiefel, Philipp, Schmidt-Emrich, Sabrina, Maniura-Weber, Katharina, Ren, Qun
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4337318/
https://ncbi.nlm.nih.gov/pubmed/25881030
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12866-015-0376-x
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