Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture

Primary cultured Cerebellar Granule Neurons (CGNs) have been widely used as an in vitro model in neuroscience and neuropharmacology research. However, the co-existence of glial cells and neurons in CGN culture might lead to biases in the accurate assessment of neuronal viability. Fluorescein diaceta...

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Опубликовано в: :J Vis Exp
Главные авторы: Jiajia, Lin, Shinghung, Mak, Jiacheng, Zheng, Jialing, Wang, Dilin, Xu, Shengquan, Hu, Zaijun, Zhang, Qinwen, Wang, Yifan, Han, Wei, Cui
Формат: Artigo
Язык:Inglês
Опубликовано: MyJove Corporation 2017
Предметы:
Neuroscience
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5607932/
https://ncbi.nlm.nih.gov/pubmed/28518109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55442
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