Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture

Primary cultured Cerebellar Granule Neurons (CGNs) have been widely used as an in vitro model in neuroscience and neuropharmacology research. However, the co-existence of glial cells and neurons in CGN culture might lead to biases in the accurate assessment of neuronal viability. Fluorescein diaceta...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Jiajia, Lin, Shinghung, Mak, Jiacheng, Zheng, Jialing, Wang, Dilin, Xu, Shengquan, Hu, Zaijun, Zhang, Qinwen, Wang, Yifan, Han, Wei, Cui
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2017
Subjects:
Neuroscience
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5607932/
https://ncbi.nlm.nih.gov/pubmed/28518109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55442
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