Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture

Primary cultured Cerebellar Granule Neurons (CGNs) have been widely used as an in vitro model in neuroscience and neuropharmacology research. However, the co-existence of glial cells and neurons in CGN culture might lead to biases in the accurate assessment of neuronal viability. Fluorescein diaceta...

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Jiajia, Lin, Shinghung, Mak, Jiacheng, Zheng, Jialing, Wang, Dilin, Xu, Shengquan, Hu, Zaijun, Zhang, Qinwen, Wang, Yifan, Han, Wei, Cui
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2017
Schlagworte:
Neuroscience
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5607932/
https://ncbi.nlm.nih.gov/pubmed/28518109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55442
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