Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture

Primary cultured Cerebellar Granule Neurons (CGNs) have been widely used as an in vitro model in neuroscience and neuropharmacology research. However, the co-existence of glial cells and neurons in CGN culture might lead to biases in the accurate assessment of neuronal viability. Fluorescein diaceta...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Jiajia, Lin, Shinghung, Mak, Jiacheng, Zheng, Jialing, Wang, Dilin, Xu, Shengquan, Hu, Zaijun, Zhang, Qinwen, Wang, Yifan, Han, Wei, Cui
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Neuroscience
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5607932/
https://ncbi.nlm.nih.gov/pubmed/28518109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55442
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