krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells

BACKGROUND: CRISPR/Cas9 system is a powerful tool for knocking out genes in cells. However, genes essential for cell survival cannot be directly knocked out. Traditionally, generation of conditional knockout cells requires multiple steps. RESULTS: In this study, we developed an easy and efficient st...

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Dettagli Bibliografici
Pubblicato in:J Biol Eng
Autori principali: Wang, Bei, Wang, Zishi, Wang, Daqi, Zhang, Baolong, Ong, Sang-Ging, Li, Mingqing, Yu, Wenqiang, Wang, Yongming
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2019
Soggetti:
Research
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6480908/
https://ncbi.nlm.nih.gov/pubmed/31049076
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13036-019-0150-y
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