An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells

Human pluripotent stem cells (hPSCs) represent a unique opportunity for understanding the molecular mechanisms underlying complex traits and diseases. CRISPR/Cas9 is a powerful tool to introduce genetic mutations into the hPSCs for loss-of-function studies. Here, we developed an episomal vector-base...

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Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Sci Rep
Egile Nagusiak: Xie, Yifang, Wang, Daqi, Lan, Feng, Wei, Gang, Ni, Ting, Chai, Renjie, Liu, Dong, Hu, Shijun, Li, Mingqing, Li, Dajin, Wang, Hongyan, Wang, Yongming
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Nature Publishing Group UK 2017
Gaiak:
Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC5443789/
https://ncbi.nlm.nih.gov/pubmed/28539611
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-02456-y
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