Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1

Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. To overcome this limitation, we develop the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in vivo approach to study DVL conformation in living c...

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Publicado no:Nat Commun
Main Authors: Harnoš, Jakub, Cañizal, Maria Consuelo Alonso, Jurásek, Miroslav, Kumar, Jitender, Holler, Cornelia, Schambony, Alexandra, Hanáková, Kateřina, Bernatík, Ondřej, Zdráhal, Zbyněk, Gömöryová, Kristína, Gybeľ, Tomáš, Radaszkiewicz, Tomasz Witold, Kravec, Marek, Trantírek, Lukáš, Ryneš, Jan, Dave, Zankruti, Fernández-Llamazares, Ana Iris, Vácha, Robert, Tripsianes, Konstantinos, Hoffmann, Carsten, Bryja, Vítězslav
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6472409/
https://ncbi.nlm.nih.gov/pubmed/31000703
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-09651-7
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