Fluorescent nucleic acid probe in droplets for bacterial sorting (FNAP-sort) as a high-throughput screening method for environmental bacteria with various growth rates

We have developed a new method for selectively sorting droplets containing growing bacteria using a fluorescence resonance energy transfer (FRET)-based RNA probe. Bacteria and the FRET-based RNA probe are encapsulated into nanoliter-scale droplets, which are incubated to allow for cell growth. The F...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Ota, Yuri, Saito, Kanako, Takagi, Taeko, Matsukura, Satoko, Morita, Masamune, Tsuneda, Satoshi, Noda, Naohiro
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2019
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6469844/
https://ncbi.nlm.nih.gov/pubmed/30995251
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0214533
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