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Synthetic spike-in standards for high-throughput 16S rRNA gene amplicon sequencing

High-throughput sequencing of 16S rRNA gene amplicons (16S-seq) has become a widely deployed method for profiling complex microbial communities but technical pitfalls related to data reliability and quantification remain to be fully addressed. In this work, we have developed and implemented a set of...

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Vydáno v:Nucleic Acids Res
Hlavní autoři: Tourlousse, Dieter M., Yoshiike, Satowa, Ohashi, Akiko, Matsukura, Satoko, Noda, Naohiro, Sekiguchi, Yuji
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5389483/
https://ncbi.nlm.nih.gov/pubmed/27980100
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw984
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