Dual expression of CCA-adding enzyme and RNase T in Escherichia coli generates a distinct cca growth phenotype with diverse applications

Correct synthesis and maintenance of functional tRNA 3′-CCA-ends is a crucial prerequisite for aminoacylation and must be achieved by the phylogenetically diverse group of tRNA nucleotidyltransferases. While numerous reports on the in vitro characterization exist, robust analysis under in vivo condi...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Wellner, Karolin, Pöhler, Marie-Theres, Betat, Heike, Mörl, Mario
Format: Artigo
Language:Inglês
Published: Oxford University Press 2019
Subjects:
Nucleic Acid Enzymes
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6468291/
https://ncbi.nlm.nih.gov/pubmed/30828718
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz133
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