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Dual expression of CCA-adding enzyme and RNase T in Escherichia coli generates a distinct cca growth phenotype with diverse applications

Correct synthesis and maintenance of functional tRNA 3′-CCA-ends is a crucial prerequisite for aminoacylation and must be achieved by the phylogenetically diverse group of tRNA nucleotidyltransferases. While numerous reports on the in vitro characterization exist, robust analysis under in vivo condi...

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Bibliografische gegevens
Gepubliceerd in:Nucleic Acids Res
Hoofdauteurs: Wellner, Karolin, Pöhler, Marie-Theres, Betat, Heike, Mörl, Mario
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2019
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6468291/
https://ncbi.nlm.nih.gov/pubmed/30828718
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz133
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