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Detection of DNA point mutations and mRNA expression levels by rolling circle amplification in individual cells
Rolling circle amplification has been useful for detecting point mutations in isolated nucleic acids, but its application in cytological preparations has been problematic. By pretreating cells with a combination of restriction enzymes and exonucleases, we demonstrate that rolling circle amplificatio...
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| 主要な著者: | , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
The National Academy of Sciences
2001
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC64666/ https://ncbi.nlm.nih.gov/pubmed/11724932 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.251383598 |
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