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Detection of HIV cDNA Point Mutations with Rolling-Circle Amplification Arrays
In this paper we describe an isothermal rolling-circle amplification (RCA) protocol to detect gene point mutations on chips. The method is based on an allele-specific oligonucleotide circularization mediated by a special DNA ligase. The probe is circularized when perfect complementary sequences betw...
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| Publicado no: | Molecules |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Molecular Diversity Preservation International
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6256933/ https://ncbi.nlm.nih.gov/pubmed/20335932 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules15020619 |
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