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Single Amino Acid Variant Discovery in Small Numbers of Cells

We have performed deep proteomic profiling down to as few as 9 Panc-1 cells using sample fractionation, TMT multiplexing, and a carrier/reference strategy. Off line fractionation of the TMT-labeled sample pooled with TMT-labeled carrier Panc-1 whole cell proteome was achieved using alkaline reversed...

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Detalhes bibliográficos
Publicado no:J Proteome Res
Main Authors: Tan, Zhijing, Yi, Xinpei, Carruthers, Nicholas J., Stemmer, Paul M., Lubman, David M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6465122/
https://ncbi.nlm.nih.gov/pubmed/30404448
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jproteome.8b00694
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