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CRISPR-interference-based modulation of mobile genetic elements in bacteria

Spontaneous mutagenesis of synthetic genetic constructs by mobile genetic elements frequently results in the rapid loss of engineered functions. Previous efforts to minimize such mutations required the exceedingly time-consuming manipulation of bacterial chromosomes and the complete removal of inser...

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Publicat a:Synth Biol (Oxf)
Autors principals: Nyerges, Ákos, Bálint, Balázs, Cseklye, Judit, Nagy, István, Pál, Csaba, Fehér, Tamás
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6462304/
https://ncbi.nlm.nih.gov/pubmed/31008359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/synbio/ysz008
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