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Resurrecting the bacterial tyrosyl-tRNA synthetase/tRNA pair for expanding the genetic code of both E. coli and eukaryotes

The bacteria-derived tyrosyl-tRNA synthetase (TyrRS)/tRNA pair was first used for unnatural amino acid (Uaa) mutagenesis in eukaryotic cells over 15 years ago. It provides an ideal platform to genetically encode numerous useful Uaas in eukaryotes. However, this pair has been engineered to charge onl...

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Veröffentlicht in:Cell Chem Biol
Hauptverfasser: Italia, James S., Latour, Christopher, Wrobel, Chester J. J., Chatterjee, Abhishek
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6456809/
https://ncbi.nlm.nih.gov/pubmed/30078635
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2018.07.002
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