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TYW1: A radical SAM enzyme involved in the biosynthesis of wybutosine bases

Transfer RNA is extensively modified by the actions of a variety of enzymes. The radical S-adenosyl-l-methionine enzyme TYW1 modifies tRNA(Phe) forming the characteristic tricyclic ring via the condensation of carbons 2 and 3 of pyruvate. This chapter details methods that are required for studies of...

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Publicado en:Methods Enzymol
Main Authors: Young, Anthony P., Bandarian, Vahe
Formato: Artigo
Idioma:Inglês
Publicado: 2018
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6448148/
https://ncbi.nlm.nih.gov/pubmed/30097090
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2018.04.024
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