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A Pipeline for Faecal Host DNA Analysis by Absolute Quantification of LINE-1 and Mitochondrial Genomic Elements Using ddPCR

Stool contains DNA shed from cells of the gastrointestinal (GI) tract and has great potential as a bio-specimen for non-invasive, nucleic acid-based detection of GI diseases. Whereas methods for studying faecal microbiome DNA are plentiful, there is a lack of well-characterised procedures for stabil...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: He, Kuang, Fujiwara, Hideaki, Zajac, Cynthia, Sandford, Erin, Reddy, Pavan, Choi, Sung Won, Tewari, Muneesh
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6447604/
https://ncbi.nlm.nih.gov/pubmed/30944341
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-41753-6
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