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In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain
High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 50 neurons...
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| Publicado no: | Nat Methods |
|---|---|
| Main Authors: | , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6441362/ https://ncbi.nlm.nih.gov/pubmed/28218900 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4183 |
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