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In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain

High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 50 neurons...

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Podrobná bibliografie
Vydáno v:Nat Methods
Hlavní autoři: Ouzounov, Dimitre G, Wang, Tianyu, Wang, Mengran, Feng, Danielle D, Horton, Nicholas G, Cruz-Hernández, Jean C, Cheng, Yu-Ting, Reimer, Jacob, Tolias, Andreas S, Nishimura, Nozomi, Xu, Chris
Médium: Artigo
Jazyk:Inglês
Vydáno: 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6441362/
https://ncbi.nlm.nih.gov/pubmed/28218900
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4183
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