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GCaMP6 ΔF/F dependence on the excitation wavelength in 3-photon and 2-photon microscopy of mouse brain activity
A fundamental challenge in calcium imaging is to minimize the excitation laser power while still maintaining a sufficient signal-to-noise ratio to distinguish individual transients in the fluorescence traces. It is important to characterize relative fluorescence (i.e., ΔF/F) dependence on the excita...
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Publicado no: | Biomed Opt Express |
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Main Authors: | , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Optical Society of America
2019
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6706025/ https://ncbi.nlm.nih.gov/pubmed/31467781 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.10.003343 |
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