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GCaMP6 ΔF/F dependence on the excitation wavelength in 3-photon and 2-photon microscopy of mouse brain activity

A fundamental challenge in calcium imaging is to minimize the excitation laser power while still maintaining a sufficient signal-to-noise ratio to distinguish individual transients in the fluorescence traces. It is important to characterize relative fluorescence (i.e., ΔF/F) dependence on the excita...

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Detalhes bibliográficos
Publicado no:Biomed Opt Express
Main Authors: Ouzounov, Dimitre G, Wang, Tianyu, Wu, Chunyan, Xu, Chris
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6706025/
https://ncbi.nlm.nih.gov/pubmed/31467781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.10.003343
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