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Unexpected insertion of carrier DNA sequences into the fission yeast genome during CRISPR–Cas9 mediated gene deletion

OBJECTIVES: The fission yeast Schizosaccharomyces pombe is predicted to encode ~ 200 proteins of < 100 amino acids, including a number of previously uncharacterised proteins that are found conserved in related Schizosaccharomyces species only. To begin an investigation of the function of four of...

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Bibliografiske detaljer
Udgivet i:BMC Res Notes
Main Authors: Longmuir, Sophie, Akhtar, Nabihah, MacNeill, Stuart A.
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6441176/
https://ncbi.nlm.nih.gov/pubmed/30925937
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-019-4228-x
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