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Cell transfection of purified cytolethal distending toxin B subunits allows comparing their nuclease activity while plasmid degradation assay does not

The Cytolethal Distending Toxin (CDT) is produced by many pathogenic bacteria. CDT is known to induce genomic DNA damage to host eukaryotic cells through its catalytic subunit, CdtB. CdtB is structurally homologous to DNase I and has a nuclease activity, dependent on several key residues. Yet some d...

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Bibliografische gegevens
Gepubliceerd in:PLoS One
Hoofdauteurs: Pons, Benoît J., Bezine, Elisabeth, Hanique, Mélissa, Guillet, Valérie, Mourey, Lionel, Chicher, Johana, Frisan, Teresa, Vignard, Julien, Mirey, Gladys
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2019
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6438463/
https://ncbi.nlm.nih.gov/pubmed/30921382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0214313
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