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Cell transfection of purified cytolethal distending toxin B subunits allows comparing their nuclease activity while plasmid degradation assay does not

The Cytolethal Distending Toxin (CDT) is produced by many pathogenic bacteria. CDT is known to induce genomic DNA damage to host eukaryotic cells through its catalytic subunit, CdtB. CdtB is structurally homologous to DNase I and has a nuclease activity, dependent on several key residues. Yet some d...

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Foilsithe in:PLoS One
Main Authors: Pons, Benoît J., Bezine, Elisabeth, Hanique, Mélissa, Guillet, Valérie, Mourey, Lionel, Chicher, Johana, Frisan, Teresa, Vignard, Julien, Mirey, Gladys
Formáid: Artigo
Teanga:Inglês
Foilsithe: Public Library of Science 2019
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6438463/
https://ncbi.nlm.nih.gov/pubmed/30921382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0214313
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