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Protein-Ligand Interaction by Ligand Titration, Fast Photochemical Oxidation of Proteins and Mass Spectrometry: LITPOMS

We report a novel method named LITPOMS (ligand titration, fast photochemical oxidation of proteins and mass spectrometry) to characterize protein-ligand binding stoichiometry, binding sites, and site-specific binding constants. The system used to test the method is melittin – calmodulin, in which th...

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Bibliographic Details
Published in:J Am Soc Mass Spectrom
Main Authors: Liu, Xiaoran Roger, Zhang, Mengru Mira, Rempel, Don L., Gross, Michael L.
Format: Artigo
Language:Inglês
Published: 2018
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6438201/
https://ncbi.nlm.nih.gov/pubmed/30484077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-018-2076-x
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