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Protein-Ligand Interaction by Ligand Titration, Fast Photochemical Oxidation of Proteins and Mass Spectrometry: LITPOMS
We report a novel method named LITPOMS (ligand titration, fast photochemical oxidation of proteins and mass spectrometry) to characterize protein-ligand binding stoichiometry, binding sites, and site-specific binding constants. The system used to test the method is melittin – calmodulin, in which th...
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| Publicado no: | J Am Soc Mass Spectrom |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6438201/ https://ncbi.nlm.nih.gov/pubmed/30484077 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-018-2076-x |
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